LOCUS       sequence-228781-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express OTX2 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_OTX2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatgtctt atcttaagca accgccttac gcagtcaatg ggctgagtct gaccacttcg
       61 ggtatggact tgctgcaccc ctccgtgggc tacccggggc cctgggcttc ttgtcccgca
      121 gccacccccc ggaaacagcg ccgggagagg acgacgttca ctcgggcgca gctagatgtg
      181 ctggaagcac tgtttgccaa gacccggtac ccagacatct tcatgcgaga ggaggtggca
      241 ctgaaaatca acttgcccga gtcgagggtg caggtatggt ttaagaatcg aagagctaag
      301 tgccgccaac aacagcaaca acagcagaat ggaggtcaaa acaaagtgag acctgccaaa
      361 aagaagacat ctccagctcg ggaagtgagt tcagagagtg gaacaagtgg ccaattcact
      421 cccccctcta gcacctcagt cccgaccatt gccagcagca gtgctcctgt gtctatctgg
      481 agcccagctt ccatctcccc actgtcagat cccttgtcca cctcctcttc ctgcatgcag
      541 aggtcctatc ccatgaccta tactcaggct tcaggttata gtcaaggata tgctggctca
      601 acttcctact ttgggggcat ggactgtgga tcatatttga cccctatgca tcaccagctt
      661 cccggaccag gggccacact cagtcccatg ggtaccaatg cagtcaccag ccatctcaat
      721 cagtccccag cttctctttc cacccaggga tatggagctt caagcttggg ttttaactca
      781 accactgatt gcttggatta taaggaccaa actgcctcct ggaagcttaa cttcaatgct
      841 gactgcttgg attataaaga tcagacatcc tcgtggaaat tccaggtttt g
//
