LOCUS       sequence-228784-        1554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express PAX7 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_PAX7_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggccc ttcccggcac ggtaccgaga atgatgcggc cggctccggg gcagaactac
       61 ccccgcacgg gattcccttt ggaagtgtcc accccgcttg gccaaggccg ggtcaatcag
      121 ctgggagggg tcttcatcaa tgggcgaccc ctgcctaacc acatccgcca caagatagtg
      181 gagatggccc accatggcat ccggccctgt gtcatctccc gacagctgcg tgtctcccac
      241 ggctgcgtct ccaagattct ttgccgctac caggagaccg ggtccatccg gcctggggcc
      301 atcggcggca gcaagcccag acaggtggcg actccggatg tagagaaaaa gattgaggag
      361 tacaagaggg aaaacccagg catgttcagc tgggagatcc gggacaggct gctgaaggat
      421 gggcactgtg accgaagcac tgtgccctca gtgagttcga ttagccgcgt gctcagaatc
      481 aagttcggga agaaagagga ggaggatgaa gcggacaaga aggaggacga cggcgaaaag
      541 aaggccaaac acagcatcga cggcatcctg ggcgacaaag ggaaccggct ggacgagggc
      601 tcggatgtgg agtcggaacc tgacctccca ctgaagcgca agcagcgacg cagtcggacc
      661 acattcacgg ccgagcagct ggaggagctg gagaaggcct ttgagaggac ccactaccca
      721 gacatataca cccgcgagga gctggcgcag aggaccaagc tgacagaggc gcgtgtgcag
      781 gtctggttca gtaaccgccg cgcccgttgg cgtaagcagg caggagccaa ccagctggcg
      841 gcgttcaacc accttctgcc aggaggcttc ccgcccaccg gcatgcccac gctgcccccc
      901 taccagctgc cggactccac ctaccccacc accaccatct cccaagatgg gggcagcact
      961 gtgcaccggc ctcagcccct gccaccgtcc accatgcacc agggcgggct ggctgcagcg
     1021 gctgcagccg ccgacaccag ctctgcctac ggagcccgcc acagcttctc cagctactct
     1081 gacagcttca tgaatccggc ggcgccctcc aaccacatga acccggtcag caacggcctg
     1141 tctcctcagg tgatgagcat cttgggcaac cccagtgcgg tgcccccgca gccacaggct
     1201 gacttctcca tctccccgct gcatggcggc ctggactcgg ccacctccat ctcagccagc
     1261 tgcagccagc gggccgactc catcaagcca ggagacagcc tgcccacctc ccaggcctac
     1321 tgcccaccca cctacagcac caccggctac agcgtggacc ccgtggccgg ctatcagtac
     1381 ggccagtacg gccagagtga gtgcctggtg ccctgggcgt cccccgtccc cattccttct
     1441 cccaccccca gggcctcctg cttgtttatg gagagctaca aggtggtgtc agggtgggga
     1501 atgtccattt cacagatgga aaaattgaag tccagccaga tggaacagtt cacc
//
