LOCUS       sequence-228787-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express POU1F1 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_POU1F1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagttgcc aagcttttac ttcggctgat acctttatac ctctgaattc tgacgcctct
       61 gcaactctgc ctctgataat gcatcacagt gctgccgagt gtctaccagt ctccaaccat
      121 gccaccaatg tgatgtctac agcaacagga cttcattatt ctgttccttc ctgtcattat
      181 ggaaaccagc catcaaccta tggagtgatg gcaggtagtt taaccccttg tctttataaa
      241 tttcctgacc acaccttgag tcatggattt cctcctatac accagcctct tctggcagag
      301 gaccccacag ctgctgattt caagcaggaa ctcaggcgga aaagtaaatt ggtggaagag
      361 ccaatagaca tggattctcc agaaatcaga gaacttgaaa agtttgccaa tgaatttaaa
      421 gtgagacgaa ttaaattagg atacacccag acaaatgttg gggaggccct ggcagctgtg
      481 catggctctg aattcagtca aacaacaatc tgccgatttg aaaatctgca gctcagcttt
      541 aaaaatgcat gcaaactgaa agcaatatta tccaaatggc tggaggaagc tgagcaagta
      601 ggagctttgt acaatgaaaa agtgggagca aatgaaagga aaagaaaacg aagaacaact
      661 ataagcattg ctgctaaaga tgctctggag agacactttg gagaacagaa taaaccttct
      721 tctcaagaga tcatgaggat ggctgaagaa ctgaatctgg agaaagaagt agtaagagtt
      781 tggttttgca accggaggca gagagaaaaa cgggtgaaaa caagtctgaa tcagagttta
      841 ttttctattt ctaaggaaca tcttgagtgc agatcaggcc tcatgggccc agctttcttg
      901 tac
//
