LOCUS       sequence-228793-        1440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express RUNX1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_RUNX1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcttcag acagcatatt tgagtcattt ccttcgtacc cacagtgctt catgagagaa
       61 tgcatacttg gaatgaatcc ttctagagac gtccacgatg ccagcacgag ccgccgcttc
      121 acgccgcctt ccaccgcgct gagcccaggc aagatgagcg aggcgttgcc gctgggcgcc
      181 ccggacgccg gcgctgccct ggccggcaag ctgaggagcg gcgaccgcag catggtggag
      241 gtgctggccg accacccggg cgagctggtg cgcaccgaca gccccaactt cctctgctcc
      301 gtgctgccta cgcactggcg ctgcaacaag accctgccca tcgctttcaa ggtggtggcc
      361 ctaggggatg ttccagatgg cactctggtc actgtgatgg ctggcaatga tgaaaactac
      421 tcggctgagc tgagaaatgc taccgcagcc atgaagaacc aggttgcaag atttaatgac
      481 ctcaggtttg tcggtcgaag tggaagaggg aaaagcttca ctctgaccat cactgtcttc
      541 acaaacccac cgcaagtcgc cacctaccac agagccatca aaatcacagt ggatgggccc
      601 cgagaacctc gaagacatcg gcagaaacta gatgatcaga ccaagcccgg gagcttgtcc
      661 ttttccgagc ggctcagtga actggagcag ctgcggcgca cagccatgag ggtcagccca
      721 caccacccag cccccacgcc caaccctcgt gcctccctga accactccac tgcctttaac
      781 cctcagcctc agagtcagat gcaggataca aggcagatcc aaccatcccc accgtggtcc
      841 tacgatcagt cctaccaata cctgggatcc attgcctctc cttctgtgca cccagcaacg
      901 cccatttcac ctggacgtgc cagcggcatg acaaccctct ctgcagaact ttccagtcga
      961 ctctcaacgg cacccgacct gacagcgttc agcgacccgc gccagttccc cgcgctgccc
     1021 tccatctccg acccccgcat gcactatcca ggcgccttca cctactcccc gacgccggtc
     1081 acctcgggca tcggcatcgg catgtcggcc atgggctcgg ccacgcgcta ccacacctac
     1141 ctgccgccgc cctaccccgg ctcgtcgcaa gcgcagggag gcccgttcca agccagctcg
     1201 ccctcctacc acctgtacta cggcgcctcg gccggctcct accagttctc catggtgggc
     1261 ggcgagcgct cgccgccgcg catcctgccg ccctgcacca acgcctccac cggctccgcg
     1321 ctgctcaacc ccagcctccc gaaccagagc gacgtggtgg aggccgaggg cagccacagc
     1381 aactccccca ccaacatggc gccctccgcg cgcctggagg aggccgtgtg gaggccctac
//
