LOCUS       sequence-228796-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SIX1 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SIX1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcgatgc tgccgtcgtt tggctttacg caggagcaag tggcgtgcgt gtgcgaggtt
       61 ctgcagcaag gcggaaacct ggagcgcctg ggcaggttcc tgtggtcact gcccgcctgc
      121 gaccacctgc acaagaacga gagcgtactc aaggccaagg cggtggtcgc cttccaccgc
      181 ggcaacttcc gtgagctcta caagatcctg gagagccacc agttctcgcc tcacaaccac
      241 cccaaactgc agcaactgtg gctgaaggcg cattacgtgg aggccgagaa gctgtgcggc
      301 cgacccctgg gcgccgtggg caaatatcgg gtgcgccgaa aatttccact gccgcgcacc
      361 atctgggacg gcgaggagac cagctactgc ttcaaggaga agtcgagggg tgtcctgcgg
      421 gagtggtacg cgcacaatcc ctacccatcg ccgcgtgaga agcgggagct ggccgaggcc
      481 accggcctca ccaccaccca ggtcagcaac tggtttaaga accggaggca aagagaccgg
      541 gccgcggagg ccaaggaaag ggagaacacc gaaaacaata actcctcctc caacaagcag
      601 aaccaactct ctcctctgga agggggcaag ccgctcatgt ccagctcaga agaggaattc
      661 tcacctcccc aaagtccaga ccagaactcg gtccttctgc tgcagggcaa tatgggccac
      721 gccaggagct caaactattc tctcccgggc ttaacagcct cgcagcccag tcacggcctg
      781 cagacccacc agcatcagct ccaagactct ctgctcggcc ccctcacctc cagtctggtg
      841 gacttggggt cc
//
