LOCUS       sequence-228799-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SIX2 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SIX2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtccatgc tgcccacctt cggcttcacg caggagcaag tggcgtgcgt gtgcgaggtg
       61 ctgcagcagg gcggcaacat cgagcggctg ggccgcttcc tgtggtcgct gcccgcctgc
      121 gagcaccttc acaagaatga aagcgtgctc aaggccaagg ccgtggtggc cttccaccgc
      181 ggcaacttcc gcgagctcta caagatcctg gagagccacc agttctcgcc gcacaaccac
      241 gccaagctgc agcagctgtg gctcaaggca cactacatcg aggcggagaa gctgcgcggc
      301 cgacccctgg gcgccgtggg caaataccgc gtgcgccgca aattcccgct gccgcgctcc
      361 atctgggacg gcgaggagac cagctactgc ttcaaggaaa agagtcgcag cgtgctgcgc
      421 gagtggtacg cgcacaaccc ctacccttca ccccgcgaga agcgtgagct gacggaggcc
      481 acgggcctca ccaccacaca ggtcagcaac tggttcaaga accggcggca gcgcgaccgg
      541 gcggccgagg ccaaggaaag ggagaacaac gagaactcca attctaacag ccacaacccg
      601 ctgaatggca gcggcaagtc ggtgttaggc agctcggagg atgagaagac tccatcgggg
      661 acgccagacc actcatcatc cagccccgca ctgctcctca gcccgccgcc ccctgggctg
      721 ccgtccctgc acagcctggg ccaccctccg ggccccagcg cagtgccagt gccggtgcca
      781 ggcggaggtg gagcggaccc actgcaacac caccatggcc tgcaggactc catcctcaac
      841 cccatgtcag ccaacctcgt ggacctgggc tcc
//
