LOCUS       sequence-228802-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SNAI2 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SNAI2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccgcgct ccttcctggt caagaagcat ttcaacgcct ccaaaaagcc aaactacagc
       61 gaactggaca cacatacagt gattatttcc ccgtatctct atgagagtta ctccatgcct
      121 gtcataccac aaccagagat cctcagctca ggagcataca gccccatcac tgtgtggact
      181 accgctgctc cattccacgc ccagctaccc aatggcctct ctcctctttc cggatactcc
      241 tcatctttgg ggcgagtgag tccccctcct ccatctgaca cctcctccaa ggaccacagt
      301 ggctcagaaa gccccattag tgatgaagag gaaagactac agtccaagct ttcagacccc
      361 catgccattg aagctgaaaa gtttcagtgc aatttatgca ataagaccta ttcaactttt
      421 tctgggctgg ccaaacataa gcagctgcac tgcgatgccc agtctagaaa atctttcagc
      481 tgtaaatact gtgacaagga atatgtgagc ctgggcgccc tgaagatgca tattcggacc
      541 cacacattac cttgtgtttg caagatctgc ggcaaggcgt tttccagacc ctggttgctt
      601 caaggacaca ttagaactca cacgggggag aagccttttt cttgccctca ctgcaacaga
      661 gcatttgcag acaggtcaaa tctgagggct catctgcaga cccattctga tgtaaagaaa
      721 taccagtgca aaaactgctc caaaaccttc tccagaatgt ctctcctgca caaacatgag
      781 gaatctggct gctgtgtagc acac
//
