LOCUS       sequence-228805-        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SOX10 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SOX10_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggagg agcaggacct atcggaggtg gagctgagcc ccgtgggctc ggaggagccc
       61 cgctgcctgt ccccggggag cgcgccctcg ctagggcccg acggcggcgg cggcggatcg
      121 ggcctgcgag ccagcccggg gccaggcgag ctgggcaagg tcaagaagga gcagcaggac
      181 ggcgaggcgg acgatgacaa gttccccgtg tgcatccgcg aggccgtcag ccaggtgctc
      241 agcggctacg actggacgct ggtgcccatg cccgtgcgcg tcaacggcgc cagcaaaagc
      301 aagccgcacg tcaagcggcc catgaacgcc ttcatggtgt gggctcaggc agcgcgcagg
      361 aagctcgcgg accagtaccc gcacctgcac aacgctgagc tcagcaagac gctgggcaag
      421 ctctggaggc tgctgaacga aagtgacaag cgccccttca tcgaggaggc tgagcggctc
      481 cgtatgcagc acaagaaaga ccacccggac tacaagtacc agcccaggcg gcggaagaac
      541 gggaaggccg cccagggcga ggcggagtgc cccggtgggg aggccgagca aggtgggacc
      601 gccgccatcc aggcccacta caagagcgcc cacttggacc accggcaccc aggagagggc
      661 tcccccatgt cagatgggaa ccccgagcac ccctcaggcc agagccatgg cccacccacc
      721 cctccaacca ccccgaagac agagctgcag tcgggcaagg cagacccgaa gcgggacggg
      781 cgctccatgg gggagggcgg gaagcctcac atcgacttcg gcaacgtgga cattggtgag
      841 atcagccacg aggtaatgtc caacatggag acctttgatg tggctgagtt ggaccagtac
      901 ctgccgccca atgggcaccc aggccatgtg agcagctact cagcagccgg ctatgggctg
      961 ggcagtgccc tggccgtggc cagtggacac tccgcctgga tctccaagcc accaggcgtg
     1021 gctctgccca cggtctcacc acctggtgtg gatgccaaag cccaggtgaa gacagagacc
     1081 gcggggcccc aggggccccc acactacacc gaccagccat ccacctcaca gatcgcctac
     1141 acctccctca gcctgcccca ctatggctca gccttcccct ccatctcccg cccccagttt
     1201 gactactctg accatcagcc ctcaggaccc tattatggcc actcgggcca ggcctctggc
     1261 ctctactcgg ccttctccta tatggggccc tcgcagcggc ccctctacac ggccatctct
     1321 gaccccagcc cctcagggcc ccagtcccac agccccacac actgggagca gccagtatat
     1381 acgacactgt cccggccc
//
