LOCUS       sequence-228808-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SOX2 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SOX2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc
       61 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc
      121 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc
      181 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa
      241 cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg
      301 cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg
      361 aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg
      421 agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac
      481 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac
      541 ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac
      601 gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
      661 cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg
      721 ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac
      781 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc
      841 gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc
      901 ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat g
//
