LOCUS       sequence-228814-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SPI1 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SPI1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttacagg cgtgcaaaat ggaagggttt cccctcgtcc cccctccatc agaagacctg
       61 gtgccctatg acacggatct ataccaacgc caaacgcacg agtattaccc ctatctcagc
      121 agtgatgggg agagccatag cgaccattac tgggacttcc acccccacca cgtgcacagc
      181 gagttcgaga gcttcgccga gaacaacttc acggagctcc agagcgtgca gcccccgcag
      241 ctgcagcagc tctaccgcca catggagctg gagcagatgc acgtcctcga tacccccatg
      301 gtgccacccc atcccagtct tggccaccag gtctcctacc tgccccggat gtgcctccag
      361 tacccatccc tgtccccagc ccagcccagc tcagatgagg aggagggcga gcggcagagc
      421 cccccactgg aggtgtctga cggcgaggcg gatggcctgg agcccgggcc tgggctcctg
      481 cctggggaga caggcagcaa gaagaagatc cgcctgtacc agttcctgtt ggacctgctc
      541 cgcagcggcg acatgaagga cagcatctgg tgggtggaca aggacaaggg caccttccag
      601 ttctcgtcca agcacaagga ggcgctggcg caccgctggg gcatccagaa gggcaaccgc
      661 aagaagatga cctaccagaa gatggcgcgc gcgctgcgca actacggcaa gacgggcgag
      721 gtcaagaagg tgaagaagaa gctcacctac cagttcagcg gcgaagtgct gggccgcggg
      781 ggcctggccg agcggcgcca cccgccccac
//
