LOCUS       sequence-228817-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SPIB in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SPIB_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcgccc tggaggctgc acagctcgac gggccacact tcagctgtct gtacccagat
       61 ggcgtcttct atgacctgga cagctgcaag cattccagct accctgattc agagggggct
      121 cctgactccc tgtgggactg gactgtggcc ccacctgtcc cagccacccc ctatgaagcc
      181 ttcgacccgg cagcagccgc ttttagccac ccccaggctg cccagctctg ctacgaaccc
      241 cccacctaca gccctgcagg gaacctcgaa ctggccccca gcctggaggc cccggggcct
      301 ggcctccccg cataccccac ggagaacttc gctagccaga ccctggttcc cccggcatat
      361 gccccgtacc ccagccctgt gctatcagag gaggaagact taccgttgga cagccctgcc
      421 ctggaggtct cggacagcga gtcggatgag gccctcgtgg ctggccccga ggggaaggga
      481 tccgaggcag ggactcgcaa gaagctgcgc ctgtaccagt tcctgctggg gctactgacg
      541 cgcggggaca tgcgtgagtg cgtgtggtgg gtggagccag gcgccggcgt cttccagttc
      601 tcctccaagc acaaggaact cctggcgcgc cgctggggcc agcagaaggg gaaccgcaag
      661 cgcatgacct accagaagct ggcgcgcgcc ctccgaaact acgccaagac cggcgagatc
      721 cgcaaggtca agcgcaagct cacctaccag ttcgacagcg cgctgctgcc tgcagtccgc
      781 cgggccttg
//
