LOCUS       sequence-228823-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SRY in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SRY_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta cagtccagct
       61 gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac tgaaagctgt
      121 aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca ggatagagtg
      181 aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa gatggctcta
      241 gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca gtggaaaatg
      301 cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca ggccatgcac
      361 agagagaaat acccgaatta taagtatcga cctcgtcgga aggcgaagat gctgccgaag
      421 aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt gcaactggac
      481 aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga gcaccagcta
      541 ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg ctacagccac
      601 tggacaaagc tg
//
