LOCUS       sequence-228826-        1557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express TBX5 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_TBX5_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccgacg cagacgaggg ctttggcctg gcgcacacgc ctctggagcc tgacgcaaaa
       61 gacctgccct gcgattcgaa acccgagagc gcgctcgggg cccccagcaa gtccccgtcg
      121 tccccgcagg ccgccttcac ccagcagggc atggagggaa tcaaagtgtt tctccatgaa
      181 agagaactgt ggctaaaatt ccacgaagtg ggcacggaaa tgatcataac caaggctgga
      241 aggcggatgt ttcccagtta caaagtgaag gtgacgggcc ttaatcccaa aacgaagtac
      301 attcttctca tggacattgt acctgccgac gatcacagat acaaattcgc agataataaa
      361 tggtctgtga cgggcaaagc tgagcccgcc atgcctggcc gcctgtacgt gcacccagac
      421 tcccccgcca ccggggcgca ttggatgagg cagctcgtct ccttccagaa actcaagctc
      481 accaacaacc acctggaccc atttgggcat attattctaa attccatgca caaataccag
      541 cctagattac acatcgtgaa agcggatgaa aataatggat ttggctcaaa aaatacagcg
      601 ttctgcactc acgtctttcc tgagactgcg tttatagcag tgacttccta ccagaaccac
      661 aagatcacgc aattaaagat tgagaataat ccctttgcca aaggatttcg gggcagtgat
      721 gacatggagc tgcacagaat gtcaagaatg caaagtaaag aatatcccgt ggtccccagg
      781 agcaccgtga ggcaaaaagt ggcctccaac cacagtcctt tcagcagcga gtctcgagct
      841 ctctccacct catccaattt ggggtcccaa taccagtgtg agaatggtgt ttccggcccc
      901 tcccaggacc tcctgcctcc acccaaccca tacccactgc cccaggagca tagccaaatt
      961 taccattgta ccaagaggaa agaggaagaa tgttccacca cagaccatcc ctataagaag
     1021 ccctacatgg agacatcacc cagtgaagaa gattccttct accgctctag ctatccacag
     1081 cagcagggcc tgggtgcctc ctacaggaca gagtcggcac agcggcaagc ttgcatgtat
     1141 gccagctctg cgccccccag cgagcctgtg cccagcctag aggacatcag ctgcaacacg
     1201 tggccaagca tgccttccta cagcagctgc accgtcacca ccgtgcagcc catggacagg
     1261 ctaccctacc agcacttctc cgctcacttc acctcggggc ccctggtccc tcggctggct
     1321 ggcatggcca accatggctc cccacagctg ggagagggaa tgttccagca ccagacctcc
     1381 gtggcccacc agcctgtggt caggcagtgt gggcctcaga ctggcctgca gtcccctggc
     1441 acccttcagc cccctgagtt cctctactct catggcgtgc caaggactct atcccctcat
     1501 cagtaccact ctgtgcacgg agttggcatg gtgccagagt ggagcgacaa tagcttg
//
