LOCUS       sequence-228829-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express TFAP2C in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_TFAP2C_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttgtgga aaataaccga taatgtcaag tacgaagagg actgcgagga tcgccacgac
       61 gggagcagca atgggaatcc gcgggtcccc cacctctcct ccgccgggca gcacctctac
      121 agccccgcgc cacccctctc ccacactgga gtcgccgaat atcagccgcc accctacttt
      181 ccccctccct accagcagct ggcctactcc cagtcggccg acccctactc gcatctgggg
      241 gaagcgtacg ccgccgccat caaccccctg caccagccgg cgcccacagg cagccagcag
      301 caggcctggc ccggccgcca gagccaggag ggagcggggc tgccctcgca ccacgggcgc
      361 ccggccggcc tactgcccca cctctccggg ctggaggcgg gcgcggtgag cgcccgcagg
      421 gatgcctacc gccgctccga cctgctgctg ccccacgcac acgccctgga tgccgcgggc
      481 ctggccgaga acctggggct ccacgacatg cctcaccaga tggacgaggt gcagaatgtc
      541 gacgaccagc acctgttgct gcacgatcag acagtcattc gcaaaggtcc catttccatg
      601 accaagaacc ctctgaacct cccctgtcag aaggagctgg tgggggccgt aatgaacccc
      661 actgaggtct tctgctcagt ccctggaaga ttgtcgctcc tcagctctac gtctaaatac
      721 aaagtgacag tggctgaagt acagaggcga ctgtccccac ctgaatgctt aaatgcctcg
      781 ttactgggag gtgttctcag aagagccaaa tcgaaaaatg gaggccggtc cttgcgggag
      841 aagttggaca agattgggtt gaatcttccg gccgggaggc ggaaagccgc tcatgtgact
      901 ctcctgacat ccttagtaga aggtgaagct gttcatttgg ctagggactt tgcctatgtc
      961 tgtgaagccg aatttcctag taaaccagtg gcagaatatt taaccagacc tcatcttgga
     1021 ggacgaaatg agatggcagc taggaagaac atgctattgg cggcccagca actgtgtaaa
     1081 gaattcacag aacttctcag ccaagaccgg acaccccatg ggaccagcag gctcgcccca
     1141 gtcttggaga cgaacataca gaactgcttg tctcatttca gcctgattac ccacgggttt
     1201 ggcagccagg ccatctgtgc cgcggtgtct gccctgcaga actacatcaa agaagccctg
     1261 attgtcatag acaaatccta catgaaccct ggagaccaga gtccagctga ttctaacaaa
     1321 accctggaga aaatggagaa acacaggaaa
//
