LOCUS       sequence-228835-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF2 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccctta gtgaacccat tcttcccagc ttttccacgt tcgcgtctcc ttgccgagag
       61 agaggccttc aggaaaggtg gccgagggct gaacccgagt ctggaggtac ggatgatgat
      121 cttaacagtg tgctcgattt catactctca atgggactgg acgggctggg agcggaggca
      181 gctcctgaac caccaccacc ccctccgccc ccagcgtttt actacccgga gccaggtgcg
      241 ccgccgccat attcagcccc ggcgggtggc ttggtgtccg agctcctccg gcctgaattg
      301 gatgccccgc tcggcccggc gctgcatggt agatttctgc tcgcgcctcc gggtcgactc
      361 gttaaggctg aacctcctga ggctgatggt ggaggtggct acggatgtgc ccccgggctt
      421 acccgaggac cgagaggtct taagcgggaa ggggcacctg gcccggctgc aagctgtatg
      481 cgggggcccg gtgggaggcc tcccccgccc cctgatacac ccccccttag tccagatgga
      541 ccagctcgac ttcccgcacc tggccccaga gcgagtttcc cccctccatt tggaggaccg
      601 gggtttggcg ccccaggtcc tggacttcac tacgcccctc ctgccccccc agcttttggt
      661 cttttcgacg atgctgctgc tgccgcagca gccttgggcc ttgcgccgcc cgcagccagg
      721 ggactgctca cgccaccggc aagccccctg gagctccttg aagccaagcc gaagcgagga
      781 cgcagatcat ggccgcgcaa gcggacagct acgcatacct gctcatatgc gggctgcgga
      841 aaaacctaca caaagagttc acaccttaaa gcgcaccttc gcacacacac aggcgagaaa
      901 ccatatcatt gtaactggga cggatgtgga tggaaatttg ctcggtctga tgagcttacg
      961 agacattatc gaaagcatac cggacatcgg ccctttcaat gccatctttg tgacagagct
     1021 ttttcccggt ctgaccacct cgctctgcac atgaagaggc acatg
//
