LOCUS       sequence-228838-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF3 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcatgt ttgacccagt tcctgtcaag caagaggcca tggaccctgt ctcagtgtca
       61 tacccatcta attacatgga atccatgaag cctaacaagt atggggtcat ctactccaca
      121 ccattgcctg agaagttctt tcagacccca gaaggtctgt cgcacggaat acagatggag
      181 ccagtggacc tcacggtgaa caagcggagt tcaccccctt cggctgggaa ttcgccctcc
      241 tctctgaagt tcccgtcctc acaccggaga gcctcgcctg ggttgagcat gccttcttcc
      301 agcccaccga taaaaaaata ctcaccccct tctccaggcg tgcagccctt cggcgtgccg
      361 ctgtccatgc caccagtgat ggcagctgcc ctctcgcggc atggaatacg gagcccgggg
      421 atcctgcccg tcatccagcc ggtggtggtg cagcccgtcc cctttatgta cacaagtcac
      481 ctccagcagc ctctcatggt ctccttatcg gaggagatgg aaaattccag tagtagcatg
      541 caagtacctg taattgaatc atatgagaag cctatatcac agaaaaaaat taaaatagaa
      601 cctgggatcg aaccacagag gacagattat tatcctgaag aaatgtcacc ccccttaatg
      661 aactcagtgt cccccccgca agcattgttg caagagaatc acccttcggt catcgtgcag
      721 cctgggaaga gacctttacc tgtggaatcc ccggatactc aaaggaagcg gaggatacac
      781 agatgtgatt atgatggatg caacaaagtg tacactaaaa gctcccactt gaaagcacac
      841 agaagaacac acacaggaga aaaaccctac aaatgtacat gggaagggtg cacatggaag
      901 tttgctcggt ctgatgaact aacaagacat ttccgaaaac atactggaat caaacctttc
      961 cagtgcccgg actgtgaccg cagcttctcc cgttctgacc atcttgccct ccataggaaa
     1021 cgccacatgc tagtc
//
