LOCUS       sequence-228841-        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF5 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF5_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctacaa gggtgctgag catgagcgcc cgcctgggac ccgtgcccca gccgccggcg
       61 ccgcaggacg agccggtgtt cgcgcagctc aagccggtgc tgggcgccgc gaatccggcc
      121 cgcgacgcgg cgctcttccc cggcgaggag ctgaagcacg cgcaccaccg cccgcaggcg
      181 cagcccgcgc ccgcgcaggc cccgcagccg gcccagccgc ccgccaccgg cccgcggctg
      241 cctccagagg acctggtcca gacaagatgt gaaatggaga agtatctgac acctcagctt
      301 cctccagttc ctataattcc agagcataaa aagtatagac gagacagtgc ctcagtcgta
      361 gaccagttct tcactgacac tgaagggtta ccttacagta tcaacatgaa cgtcttcctc
      421 cctgacatca ctcacctgag aactggcctc tacaaatccc agagaccgtg cgtaacacac
      481 atcaagacag aacctgttgc cattttcagc caccagagtg aaacgactgc ccctcctccg
      541 gccccgaccc aggccctccc tgagttcacc agtatattca gctcacacca gaccgcagct
      601 ccagaggtga acaatatttt catcaaacaa gaacttccta caccagatct tcatctttct
      661 gtccctaccc agcagggcca cctgtaccag ctactgaata caccggatct agatatgccc
      721 agttctacaa atcagacagc agcaatggac actcttaatg tttctatgtc agctgccatg
      781 gcaggcctta acacacacac ctctgctgtt ccgcagactg cagtgaaaca attccagggc
      841 atgccccctt gcacatacac aatgccaagt cagtttcttc cacaacaggc cacttacttt
      901 cccccgtcac caccaagctc agagcctgga agtccagata gacaagcaga gatgctccag
      961 aatttaaccc cacctccatc ctatgctgct acaattgctt ctaaactggc aattcacaat
     1021 ccaaatttac ccaccaccct gccagttaac tcacaaaaca tccaacctgt cagatacaat
     1081 agaaggagta accccgattt ggagaaacga cgcatccact actgcgatta ccctggttgc
     1141 acaaaagttt ataccaagtc ttctcattta aaagctcacc tgaggactca cactggtgaa
     1201 aagccataca agtgtacctg ggaaggctgc gactggaggt tcgcgcgatc ggatgagctg
     1261 acccgccact accggaagca cacaggcgcc aagcccttcc agtgcggggt gtgcaaccgc
     1321 agcttctcgc gctctgacca cctggccctg catatgaaga ggcaccagaa c
//
