LOCUS       sequence-228844-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF6 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF6_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacgtgc tccccatgtg cagcatcttc caggagctcc agatcgtgca cgagaccggc
       61 tacttctcgg cgctgccgtc tctggaggag tactggcaac agacctgcct agagctggaa
      121 cgttacctcc agagcgagcc ctgctatgtt tcagcctcag aaatcaaatt tgacagccag
      181 gaagatctgt ggaccaaaat cattctggct cgggagaaaa aggaggaatc cgaactgaag
      241 atatcttcca gtcctccaga ggacactctc atcagcccga gcttttgtta caacttagag
      301 accaacagcc tgaactcaga tgtcagcagc gaatcctctg acagctccga ggaactttct
      361 cccacggcca agtttacctc cgaccccatt ggcgaagttt tggtcagctc gggaaaattg
      421 agctcctctg tcacctccac gcctccatct tctccggaac tgagcaggga accttctcaa
      481 ctgtggggtt gcgtgcccgg ggagctgccc tcgccaggga aggtgcgcag cgggacttcg
      541 gggaagccag gtgacaaggg aaatggcgat gcctcccccg acggcaggag gagggtgcac
      601 cggtgccact ttaacggctg caggaaagtt tacaccaaaa gctcccactt gaaagcacac
      661 cagcggacgc acacaggaga aaagccttac agatgctcat gggaagggtg tgagtggcgt
      721 tttgcaagaa gtgatgagtt aaccaggcac ttccgaaagc acaccggggc caagcctttt
      781 aaatgctccc actgtgacag gtgtttttcc aggtctgacc acctggccct gcacatgaag
      841 aggcacctc
//
