LOCUS       sequence-228847-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF7 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF7_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacgtgt tggctagtta tagtatattc caggagctac aacttgtcca cgacaccggc
       61 tacttctcag ctttaccatc cctggaggag acctggcagc agacatgcct tgaattggaa
      121 cgctacctac agacggagcc ccggaggatc tcagagacct ttggtgagga cttggactgt
      181 ttcctccacg cttcccctcc cccgtgcatt gaggaaagct tccgtcgctt agaccccctg
      241 ctgctccccg tggaagcggc catctgtgag aagagctcgg cagtggacat cttgctctct
      301 cgggacaagt tgctatctga gacctgcctc agcctccagc cggccagctc ttctctagac
      361 agctacacag ccgtcaacca ggcccagctc aacgcagtga cctcattaac gcccccatcg
      421 tcccctgagc tcagccgcca tctggtcaaa acctcacaaa ctctctctgc cgtggatggc
      481 acggtgacgt tgaaactggt ggccaagaag gctgctctca gctccgtaaa ggtgggaggg
      541 gtcgcaacag ctgcagcagc cgtgacggct gcgggggccg ttaagagtgg acagagcgac
      601 agtgaccaag gagggctagg ggctgaagca tgtcccgaaa acaagaagag ggttcaccgc
      661 tgtcagttta acgggtgccg gaaagtttat acaaaaagct cccacttaaa ggcccaccag
      721 aggactcaca caggtgagaa gccttataag tgctcatggg agggatgtga gtggcgtttt
      781 gcacgaagcg atgagctcac gaggcactac aggaaacaca caggtgcaaa gcccttcaaa
      841 tgcaaccact gcgacaggtg tttttccagg tctgaccatc ttgccctcca catgaagaga
      901 catatc
//
