LOCUS       sequence-228850-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF8 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF8_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtcgata tggataaact cataaacaac ttggaggtcc aacttaattc agaaggtggc
       61 tcaatgcagg tattcaagca ggtcactgct tctgttcgga acagagatcc ccctgagata
      121 gaatacagaa gtaatatgac ttctccaaca ctcctggatg ccaaccccat ggagaaccca
      181 gcactgttta atgacatcaa gattgagccc ccagaagaac ttttggctag tgatttcagc
      241 ctgccccaag tggaaccagt tgacctctcc tttcacaagc ccaaggctcc tctccagcct
      301 gctagcatgc tacaagctcc aatacgtccc cccaagccac agtcttctcc ccagaccctt
      361 gtggtgtcca cgtcaacatc tgacatgagc acttcagcaa acattcctac tgttctgacc
      421 ccaggctctg tcctgacctc ctctcagagc actggtagcc agcagatctt acatgtcatt
      481 cacactatcc cctcagtcag tctgccaaat aagatgggtg gcctgaagac catcccagtg
      541 gtagtgcagt ctctgcccat ggtgtatact actttgcctg cagatggggg ccctgcagcc
      601 attacagtcc cactcattgg aggagatggt aaaaatgctg gatcagtgaa agttgacccc
      661 acctccatgt ctccactgga aattccaagt gacagtgagg agagtacaat tgagagtgga
      721 tcctcagcct tgcagagtct gcagggacta cagcaagaac cagcagcaat ggcccaaatg
      781 cagggagaag agtcgcttga cttgaagaga agacggattc accaatgtga ctttgcagga
      841 tgcagcaaag tgtacaccaa aagctctcac ctgaaagctc accgcagaat ccatacagga
      901 gagaagcctt ataaatgcac ctgggatggc tgctcctgga aatttgctcg ctcagatgag
      961 ctcactcgcc atttccgcaa gcacacaggc atcaagcctt ttcggtgcac agactgcaac
     1021 cgcagctttt ctcgttctga ccacctgtcc ctgcatcgcc gtcgccatga caccatg
//
