LOCUS       sequence-228853-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF9 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF9_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtccgcgg ccgcctacat ggacttcgtg gctgcccagt gtctggtttc catttcgaac
       61 cgcgctgcgg tgccggagca tggggtcgct ccggacgccg agcggctgcg actacctgag
      121 cgcgaggtga ccaaggagca cggtgacccg ggggacacct ggaaggatta ctgcacactg
      181 gtcaccatcg ccaagagctt gttggacctg aacaagtacc gacccatcca gaccccctcc
      241 gtgtgcagcg acagtctgga aagtccagat gaggatatgg gatccgacag cgacgtgacc
      301 accgaatctg ggtcgagtcc ttcccacagc ccggaggaga gacaggatcc tggcagcgcg
      361 cccagcccgc tctccctcct ccatcctgga gtggctgcga aggggaaaca cgcctccgaa
      421 aagaggcaca agtgccccta cagtggctgt gggaaagtct atggaaaatc ctcccatctc
      481 aaagcccatt acagagtgca tacaggtgaa cggccctttc cctgcacgtg gccagactgc
      541 cttaaaaagt tctcccgctc agacgagctg acccgccact accggaccca cactggggaa
      601 aagcagttcc gctgtccgct gtgtgagaag cgcttcatga ggagtgacca cctcacaaag
      661 cacgcccggc ggcacaccga gttccacccc agcatgatca agcgatcgaa aaaggcgctg
      721 gccaacgctt tg
//
