LOCUS       sequence-228856-        1440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF10 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF10_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcaact tcggtgcctc tctccagcag actgcggagg aaagaatgga aatgatttct
       61 gaaaggccaa aagagagtat gtattcctgg aacaaaactg cagagaaaag tgattttgaa
      121 gctgtagaag cacttatgtc aatgagctgc agttggaagt ctgattttaa gaaatacgtt
      181 gaaaacagac ctgttacacc agtatctgat ttgtcagagg aagagaatct gcttccggga
      241 acacctgatt ttcatacaat cccagcattt tgtttgactc caccttacag tccttctgac
      301 tttgaaccct ctcaagtgtc aaatctgatg gcaccagcgc catctactgt acacttcaag
      361 tcactctcag atactgccaa acctcacatt gccgcacctt tcaaagagga agaaaagagc
      421 ccagtatctg cccccaaact ccccaaagct caggcaacaa gtgtgattcg tcatacagct
      481 gatgcccagc tatgtaacca ccagacctgc ccaatgaaag cagccagcat cctcaactat
      541 cagaacaatt cttttagaag aagaacccac ctaaatgttg aggctgcaag aaagaacata
      601 ccatgtgccg ctgtgtcacc aaacagatcc aaatgtgaga gaaacacagt ggcagatgtt
      661 gatgagaaag caagtgctgc actttatgac ttttctgtgc cttcctcaga gacggtcatc
      721 tgcaggtctc agccagcccc tgtgtcccca caacagaagt cagtgttggt ctctccacct
      781 gcagtatctg cagggggagt gccacctatg ccggtcatct gccagatggt tccccttcct
      841 gccaacaacc ctgttgtgac aacagtcgtt cccagcactc ctcccagcca gccaccagcc
      901 gtttgccccc ctgttgtgtt catgggcaca caagtcccca aaggcgctgt catgtttgtg
      961 gtaccccagc ccgttgtgca gagttcaaag cctccggtgg tgagcccgaa tggcaccaga
     1021 ctctctccca ttgcccctgc tcctgggttt tccccttcag cagcaaaagt cactcctcag
     1081 attgattcat caaggataag gagtcacatc tgtagccacc caggatgtgg caagacatac
     1141 tttaaaagtt cccatctgaa ggcccacacg aggacgcaca caggagaaaa gcctttcagc
     1201 tgtagctgga aaggttgtga aaggaggttt gcccgttctg atgaactgtc cagacacagg
     1261 cgaacccaca cgggtgagaa gaaatttgcg tgccccatgt gtgaccggcg gttcatgagg
     1321 agtgaccatt tgaccaagca tgcccggcgc catctatcag ccaagaagct accaaactgg
     1381 cagatggaag tgagcaagct aaatgacatt gctctacctc caacccctgc tcccacacag
//
