LOCUS       sequence-228859-        1536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF11 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF11_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcatactc ctgatttcgc tggacctgac gacgcccgag ccgtggacat tatggacatt
       61 tgtgaatcta tactcgaaag aaagagacat gattcagagc gaagtacatg ctctatcctc
      121 gagcaaacag acatggaggc ggtagaagct ctggtgtgca tgtccagttg gggtcagaga
      181 tcccagaagg gggacttgct tagaatccga ccgcttactc cagtttccga tagcggcgac
      241 gtaacaacta ctgttcatat ggacgcagcc acgcctgagc tgcccaaaga ctttcacagc
      301 ctctcaactc tttgcatcac tccaccacag tcccccgatc ttgtcgaacc atcaacccgg
      361 acccctgtta gcccgcaagt tacagattca aaggcgtgta ccgcgaccga tgttctgcag
      421 agttcagcgg ttgtagcgcg ggcattgagc ggaggggctg aacgaggtct gttgggtctt
      481 gaacccgtac cgagttctcc ttgtagagcc aagggtacta gtgttattcg gcataccggc
      541 gagagtccgg cagcttgttt ccccaccata caaaccccag actgtcgcct tagtgattcc
      601 cgggaagggg aggaacagct gttgggccac ttcgagacac ttcaagatac acacttgaca
      661 gatagcttgc tgtccaccaa cctggtgtca tgtcaacctt gtttgcacaa gtccgggggt
      721 ctccttctga ctgacaaagg tcaacaagcg ggatggcctg gcgctgtcca aacatgcagt
      781 cctaaaaact acgaaaatga tttgcctagg aaaaccacgc cgcttatcag tgtgagtgtt
      841 cccgctccac ctgtcctgtg ccagatgatc cctgtaaccg ggcaatcatc tatgttgcct
      901 gcgttcttga agcccccccc acaactgtcc gttggtactg ttcgcccgat ccttgcgcaa
      961 gcagcgcccg ccccgcaacc cgtgttcgtg gggcccgctg tcccgcaggg tgcagtcatg
     1021 ttggttcttc cccagggggc cctcccgcca ccagctccgt gtgcagcgaa tgtcatggct
     1081 gccggaaaca cgaaattgtt gccccttgca cccgctccag ttttcataac gagctcacag
     1141 aattgtgtgc cacaagtcga cttctcacga agacggaact atgtgtgctc tttcccaggt
     1201 tgcagaaaaa catatttcaa atcctctcat ctgaaagcac atcttcggac ccatacagga
     1261 gagaagcctt ttaattgtag ctgggatggc tgtgataaaa aattcgcaag aagtgatgag
     1321 ctcagtcgac atcgcaggac gcataccggg gaaaaaaaat tcgtttgtcc agtttgtgac
     1381 agaagattta tgaggtccga ccatctcacc aagcacgcgc gacgccacat gactacaaag
     1441 aaaattcctg gctggcaagc cgaggtggga aaactcaacc gaatcgcttc cgctgaatcc
     1501 cccggcagcc cgctggtaag tatgcctgcc agtgcc
//
