LOCUS       sequence-228862-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF12 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF12_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaacattc acatgaagcg caagacgata aagaacatca atacattcga gaaccgaatg
       61 ttgatgttgg atggcatgcc cgctgtacgg gtaaaaaccg agctcctgga gtctgaacaa
      121 ggatccccaa acgtccacaa ctacccggat atggaggcag tgccgctctt gctcaacaat
      181 gtgaagggag agccgcctga ggactctctc tccgtagatc atttccagac acagactgag
      241 cccgtagatc tttcaattaa caaagccaga acatctccta ctgcggtaag ttcttctccc
      301 gtaagtatga cagcaagtgc atctagtcca agttctacga gcactagcag ttcttcatct
      361 agtagacttg ctagttcacc aacggtgatc acaagtgttt ctagcgccag cagcagctca
      421 acggtactga ctcccggtcc actcgtggca agcgctagtg gcgtgggtgg ccaacaattt
      481 ctccatatta ttcaccccgt gcctccgtct agtccgatga atctccagag caacaagctt
      541 agtcacgtac ataggatccc cgtcgtcgtc cagtcagttc ccgtcgtcta cacagctgtg
      601 cgatcccctg ggaatgtcaa taatactata gttgttcctt tgcttgagga tggtaggggc
      661 catgggaaag cacagatgga cccccgcggc ttgtcaccga gacagtctaa atccgatagt
      721 gacgacgatg atttgcctaa cgtaacactg gactctgtga acgagaccgg gagtaccgct
      781 ctgtcaatcg ctagggccgt acaggaggtc cacccaagcc ctgtgtcacg agtccgaggt
      841 aacaggatga ataatcagaa atttccctgt agcatcagcc cattttctat agagtccact
      901 cggagacagc gacgaagtga atcacccgac tccagaaaaa ggaggataca tcgctgtgac
      961 tttgagggct gtaacaaggt ctacacaaaa agttcacacc tcaaggcgca tcgacggacg
     1021 catactgggg aaaaaccgta caaatgcacc tgggagggat gcacgtggaa atttgcacgc
     1081 tctgacgagt tgacacgcca ctatcgaaag catacgggcg taaagccgtt taaatgcgct
     1141 gattgcgaca ggagttttag ccgctctgat caccttgctc ttcaccggag gcgacacatg
     1201 cttgtt
//
