LOCUS       sequence-228865-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF13 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF13_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctgcgg ctgcatatgt ggatcatttt gcggctgagt gcctggtgtc aatgtctagt
       61 agagcggtgg tacacggtcc cagagaaggc ccagaatcac gcccagaggg cgccgccgtc
      121 gctgcaacac cgacgctgcc tcgggtcgag gagcgccgcg acgggaagga cagtgcgtca
      181 cttttcgtag tagcgagaat attggcagat ctgaatcaac aggctccagc acctgcgccc
      241 gctgaacgcc gggagggcgc cgctgccaga aaggccagaa caccatgccg cttgccgcca
      301 cctgcgccag aacccacaag tccaggtgcc gaaggtgcgg cggctgcccc tccttcaccg
      361 gcctggtctg aaccagaacc agaggcaggt cttgaacctg agcgcgaacc cggccctgca
      421 ggctctgggg aacctggcct gaggcagcgg gtgaggcgcg gccggagcag ggccgacctg
      481 gaatcaccgc aaaggaaaca taaatgccat tatgctggtt gcgaaaaggt ttatggaaag
      541 tcatcccacc tgaaagcaca cctccgcact cacacgggtg agcgaccttt tgcgtgttcc
      601 tggcaagact gcaataaaaa gtttgctaga tctgatgaac ttgcacggca ttatcgaact
      661 cataccggtg aaaagaagtt ctcatgccct atatgtgaga aacggttcat gcgctctgac
      721 cacttgacga aacatgcaag acgacatgct aattttcatc cggggatgtt gcagagacgg
      781 ggagggggaa gtaggactgg aagtctctcc gactattccc gatccgacgc ttcctcacca
      841 acgattagcc ccgcaagcag tccc
//
