LOCUS       sequence-228868-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF14 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF14_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcagccg cagtcgcatg ccttgattac ttcgcggccg agtgtcttgt ttccatgtca
       61 gcgggggctg tcgttcacag aagaccacca gacccggagg gagcgggagg ggcagctgga
      121 tctgaagtcg gcgcggctcc acctgaatca gcgcttcccg gccctggtcc tccaggtccc
      181 gctagcgtgc cccaactccc acaagtgcct gctccgagtc ctggagcggg cggagcagcc
      241 ccgcatctcc ttgcagcatc agtgtgggcc gatcttcgcg gaagctccgg ggagggctcc
      301 tgggaaaaca gcggagaggc cccgcgagct tcaagcggct tttccgatcc aatcccttgc
      361 agtgttcaaa ccccatgctc cgagctcgcg cccgcgtccg gagctgcggc agtgtgcgca
      421 cctgaaagct catccgatgc gccggccgtt ccatctgcgc cagctgctcc cggtgcaccc
      481 gcagcatctg gcggctttag tggtggagct cttggggcgg gtcccgcccc tgcggcggat
      541 caagctcctc gcaggcgcag tgttacgccc gcagcaaaac ggcatcaatg cccctttcct
      601 ggttgtacaa aagcatacta taagtcatcc catctcaaga gtcaccagag gacgcataca
      661 ggtgagagac cttttagctg tgactggctc gattgcgaca agaaatttac gcggagcgac
      721 gaacttgcgc ggcactaccg cactcacact ggagaaaaga ggttctcttg tcccctgtgt
      781 cccaagcagt tctcacgcag tgatcacttg acaaaacatg ctaggagaca tccaacatac
      841 catcccgaca tgatagagta tcgaggtagg cgacgcacac ctagaattga tcctccgctg
      901 actagtgaag tcgagtcaag tgccagtgga agcggaccgg gtcccgcgcc ctcatttaca
      961 acctgtctt
//
