LOCUS       sequence-228871-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF15 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF15_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtggacc acttacttcc agtggacgag aacttctcgt cgccaaaatg cccagttggg
       61 tatctgggtg ataggctggt tggccggcgg gcatatcaca tgctgccctc acccgtctct
      121 gaagatgaca gcgatgcctc cagcccctgc tcctgttcca gtcccgactc tcaagccctc
      181 tgctcctgct atggtggagg cctgggcacc gagagccagg acagcatctt ggacttccta
      241 ttgtcccagg ccacgctggg cagtggcggg ggcagcggca gtagcattgg ggccagcagt
      301 ggccccgtgg cctgggggcc ctggcgaagg gcagcggccc ctgtgaaggg ggagcatttc
      361 tgcttgcccg agtttccttt gggtgatcct gatgacgtcc cacggccctt ccagcctacc
      421 ctggaggaga ttgaagagtt tctggaggag aacatggagc ctggagtcaa ggaggtccct
      481 gagggcaaca gcaaggactt ggatgcctgc agccagctct cagctgggcc acacaagagc
      541 cacctccatc ctgggtccag cgggagagag cgctgttccc ctccaccagg tggtgccagt
      601 gcaggaggtg cccagggccc aggtgggggc cccacgcctg atggccccat cccagtgttg
      661 ctgcagatcc agcccgtgcc tgtgaagcag gaatcgggca cagggcctgc ctcccctggg
      721 caagccccag agaatgtcaa ggttgcccag ctcctggtca acatccaggg gcagaccttc
      781 gcactcgtgc cccaggtggt accctcctcc aacttgaacc tgccctccaa gtttgtgcgc
      841 attgcccctg tgcccattgc cgccaagcct gttggatcgg gacccctggg gcctggccct
      901 gccggtctcc tcatgggcca gaagttcccc aagaacccag ccgcagaact catcaaaatg
      961 cacaaatgta ctttccctgg ctgcagcaag atgtacacca aaagcagcca cctcaaggcc
     1021 cacctgcgcc ggcacacggg tgagaagccc ttcgcctgca cctggccagg ctgcggctgg
     1081 aggttctcgc gctctgacga gctgtcgcgg cacaggcgct cgcactcagg tgtgaagccg
     1141 taccagtgtc ctgtgtgcga gaagaagttc gcgcggagcg accacctctc caagcacatc
     1201 aaggtgcacc gcttcccgcg gagcagccgc tccgtgcgct ccgtgaac
//
