LOCUS       sequence-228874-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF16 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF16_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcagccg cggtcgcgtg cgtggattat tttgcagcag atgtgctgat ggcaatttca
       61 tccggtgcag tagttcatcg cggaagacca ggtcctgagg gtgcggggcc tgcggccggg
      121 ttggatgttc gcgccgcgcg cagggaagcc gcttctcccg gaacacctgg ccctcctcct
      181 cctccgccgg cggcatcagg cccgggtcct ggtgcagctg cggctcctca cctgttggca
      241 gcctccatac tggctgacct gcgagggggg ccaggcgctg cacctggtgg cgcgagtcca
      301 gcaagttcca gctccgcggc gtcctccccg agtagtgggc gagctccggg cgcggcacct
      361 tctgctgccg ctaaatcaca ccgatgccct ttcccagact gcgcgaaggc gtattataag
      421 tccagtcatt tgaaatcaca cttgaggaca cataccggcg agagaccttt tgcgtgcgac
      481 tggcagggtt gtgataagaa atttgcgaga agcgacgaac tggcccgcca tcaccgcacc
      541 cacacagggg aaaaaagatt ctcatgccca ctctgttcta agcgcttcac gcgaagcgac
      601 catcttgcaa agcacgctag gagacaccct gggttccacc ccgacctctt gcgacgacct
      661 ggcgcccggt ctactagccc gtctgactca ttgccgtgct ctctcgcagg gtcccctgct
      721 ccgagccccg caccgtcccc agctcctgcc gggctt
//
