LOCUS       sequence-228877-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF17 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF17_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtacggcc gaccgcaggc tgagatggaa caggaggctg gggagctgag ccggtggcag
       61 gcggcgcacc aggctgccca ggataacgag aactcagcgc ccatcttgaa catgtcttca
      121 tcttctggaa gctctggagt gcacacctct tggaaccaag gcctaccaag cattcagcac
      181 tttcctcaca gcgcagagat gctggggtcc cctttggtgt ctgttgaggc gccggggcag
      241 aatgtgaatg aaggggggcc acagttcagt atgccactgc ctgagcgtgg tatgagctac
      301 tgcccccaag cgactctcac tccttcccgg atgatttact gtcagagaat gtctccccct
      361 cagcaagaga tgacgatttt cagtgggccc caactaatgc ccgtaggaga gcccaatatt
      421 ccaagggtag ccaggccctt cggtgggaat ctaaggatgc cccccaatgg gctgccagtc
      481 tcggcttcca ctggaatccc aataatgtcc cacactggga accctccagt gccttaccct
      541 ggcctctcga cagtaccttc tgacgaaaca ttgttgggcc cgactgtgcc ttccactgag
      601 gcccaggcag tgctcccctc catggctcag atgttgcccc cgcaagatgc ccatgacctt
      661 gggatgcccc cagctgagtc ccagtcattg ctggttttag gatctcagga ctctcttgtc
      721 agtcagccag actctcaaga aggcccattt ctaccagagc agcccggacc tgctccacag
      781 acagtagaga agaactccag gcctcaggaa gggactggta gaaggggctc ctcagaggca
      841 aggccttact gctgcaacta cgagaactgc ggaaaagctt ataccaaacg ctcccacctc
      901 gtgagccacc agcgcaagca cacaggtgag aggccatatt cttgcaactg ggaaagttgt
      961 tcatggtctt tcttccgttc tgatgagctt agacgacata tgcgggtaca caccagatat
     1021 cgaccatata aatgtgatca gtgcagccgg gagttcatga ggtctgacca tctcaagcaa
     1081 caccagaaga ctcatcggcc gggaccctca gacccacagg ccaacaacaa caatggagag
     1141 caggacagtc ctcctgctgc tggtcct
//
