LOCUS       sequence-228880-        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MYC deltaMBI in mammalian 
            cells under control of EF1a promoter. Barcoded for transcript 
            detection in single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYC deltaMBI_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             916..942
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1186..1215
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgcccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag
       61 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg
      121 cagcccccgg cgggatcagg tagcggtagc cgccgctccg ggctctgctc gccctcctac
      181 gttgcggtca cacccttctc ccttcgggga gacaacgacg gcggtggcgg gagcttctcc
      241 acggccgacc agctggagat ggtgaccgag ctgctgggag gagacatggt gaaccagagt
      301 ttcatctgcg acccggacga cgagaccttc atcaaaaaca tcatcatcca ggactgtatg
      361 tggagcggct tctcggccgc cgccaagctc gtctcagaga agctggcctc ctaccaggct
      421 gcgcgcaaag acagcggcag cccgaacccc gcccgcggcc acagcgtctg ctccacctcc
      481 agcttgtacc tgcaggatct gagcgccgcc gcctcagagt gcatcgaccc ctcggtggtc
      541 ttcccctacc ctctcaacga cagcagctcg cccaagtcct gcgcctcgca agactccagc
      601 gccttctctc cgtcctcgga ttctctgctc tcctcgacgg agtcctcccc gcagggcagc
      661 cccgagcccc tggtgctcca tgaggagaca ccgcccacca ccagcagcga ctctgaggag
      721 gaacaagaag atgaggaaga aatcgatgtt gtttctgtgg aaaagaggca ggctcctggc
      781 aaaaggtcag agtctggatc accttctgct ggaggccaca gcaaacctcc tcacagccca
      841 ctggtcctca agaggtgcca cgtctccaca catcagcaca actacgcagc gcctccctcc
      901 actcggaagg actatcctgc tgccaagagg gtcaagttgg acagtgtcag agtcctgaga
      961 cagatcagca acaaccgaaa atgcaccagc cccaggtcct cggacaccga ggagaatgtc
     1021 aagaggcgaa cacacaacgt cttggagcgc cagaggagga acgagctaaa acggagcttt
     1081 tttgccctgc gtgaccagat cccggagttg gaaaacaatg aaaaggcccc caaggtagtt
     1141 atccttaaaa aagccacagc atacatcctg tccgtccaag cagaggagca aaagctcatt
     1201 tctgaagagg acttgttgcg gaaacgacga gaacagttga aacacaaact tgaacagcta
     1261 cggaactctt gtgcg
//
