LOCUS       sequence-228883-        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian 
            cells under control of EF1a promoter. Barcoded for transcript 
            detection in single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYC deltaMBII_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             928..954
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1198..1227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgcccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag
       61 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg
      121 cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc caccccgccc
      181 ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc
      241 tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag
      301 atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac
      361 gacgagacct tcatcaaaaa catcggatca ggtagcggtc tcgtctcaga gaagctggcc
      421 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc
      481 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac
      541 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg
      601 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc
      661 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc
      721 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg
      781 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct
      841 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca
      901 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc
      961 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc
     1021 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta
     1081 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc
     1141 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag
     1201 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa
     1261 cttgaacagc tacggaactc ttgtgcg
//
