LOCUS       sequence-228889-        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MYC deltab in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYC deltab_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             958..984
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1201..1230
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgcccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag
       61 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg
      121 cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc caccccgccc
      181 ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc
      241 tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag
      301 atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac
      361 gacgagacct tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc
      421 gccgccaagc tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc
      481 agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat
      541 ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac
      601 gacagcagct cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg
      661 gattctctgc tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc
      721 catgaggaga caccgcccac caccagcagc gactctgagg aggaacaaga agatgaggaa
      781 gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc agagtctgga
      841 tcaccttctg ctggaggcca cagcaaacct cctcacagcc cactggtcct caagaggtgc
      901 cacgtctcca cacatcagca caactacgca gcgcctccct ccactcggaa ggactatcct
      961 gctgccaaga gggtcaagtt ggacagtgtc agagtcctga gacagatcag caacaaccga
     1021 aaatgcacca gccccaggtc ctcggacacc gaggagaatg tcggatcagg tagcggtgag
     1081 ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa caatgaaaag
     1141 gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt ccaagcagag
     1201 gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca gttgaaacac
     1261 aaacttgaac agctacggaa ctcttgtgcg
//
