LOCUS       sequence-228898-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MYC deltaNTD in mammalian 
            cells under control of EF1a promoter. Barcoded for transcript 
            detection in single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYC deltaNTD_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             547..573
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             817..846
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggatcag gtagcggtct cgtctcagag aagctggcct cctaccaggc tgcgcgcaaa
       61 gacagcggca gcccgaaccc cgcccgcggc cacagcgtct gctccacctc cagcttgtac
      121 ctgcaggatc tgagcgccgc cgcctcagag tgcatcgacc cctcggtggt cttcccctac
      181 cctctcaacg acagcagctc gcccaagtcc tgcgcctcgc aagactccag cgccttctct
      241 ccgtcctcgg attctctgct ctcctcgacg gagtcctccc cgcagggcag ccccgagccc
      301 ctggtgctcc atgaggagac accgcccacc accagcagcg actctgagga ggaacaagaa
      361 gatgaggaag aaatcgatgt tgtttctgtg gaaaagaggc aggctcctgg caaaaggtca
      421 gagtctggat caccttctgc tggaggccac agcaaacctc ctcacagccc actggtcctc
      481 aagaggtgcc acgtctccac acatcagcac aactacgcag cgcctccctc cactcggaag
      541 gactatcctg ctgccaagag ggtcaagttg gacagtgtca gagtcctgag acagatcagc
      601 aacaaccgaa aatgcaccag ccccaggtcc tcggacaccg aggagaatgt caagaggcga
      661 acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg
      721 cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa
      781 aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat ttctgaagag
      841 gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct acggaactct
      901 tgtgcg
//
