LOCUS       sequence-228914-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses ERG protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Sandoval GJ, Pulice JL, Pakula H, Schenone M, Takeda DY, Pop M, 
            Boulay G, Williamson KE, McBride MJ, Pan J, St Pierre R, Hartman E, 
            Garraway LA, Carr SA, Rivera MN, Li Z, Ronco L, Hahn WC, Kadoch C
  TITLE     Binding of TMPRSS2-ERG to BAF Chromatin Remodeling Complexes 
            Mediates Prostate Oncogenesis.
  JOURNAL   Mol Cell. 2018 Aug 16;71(4):554-566.e7. doi: 
            10.1016/j.molcel.2018.06.040. Epub 2018 Aug 2.
  PUBMED    30078722
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.06.040"; journalName: "Mol Cell"; date: 
            "2018-08-16- 16"; volume: "71"; issue: "4"; pages: "554-566.e7"
COMMENT     Sequence Label: pLX304 ERG
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     540..560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    612..636
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1987..2011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2013..2054
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2096..2684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2149..2169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2567..2578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2756..2989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3067..3188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3104..3123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3158..3177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3228..3363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3383..3402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3384..3402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3412..3429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3412..3428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3421..3443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3570..4025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3657..3676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3867..3888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4051..4155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4156..4224,4225..5016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4374..4393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5187..5775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5676..5695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5929..5946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6063..6084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6099..6104,6105..6122,6123..6129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6137..6153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6142..6164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6161..6177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6161..6177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6196..6216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6198..6216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6244..6470
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6471..6651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6698..6823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7316..7549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7734..7778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7953..8453
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8372..8390
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8465..8860
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8920..9037
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcgccggctc gagggggccc ggggttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      601 tgtcgggatc aacaagtttg tacaaaaaag caggcttcac catgaccgcg tcctcctcca
      661 gcgactatgg acagacttcc aagatgagcc cacgcgtccc tcagcaggat tggctgtctc
      721 aacccccagc cagggtcacc atcaaaatgg aatgtaaccc tagccaggtg aatggctcaa
      781 ggaactctcc tgatgaatgc agtgtggcca aaggcgggaa gatggtgggc agcccagaca
      841 ccgttgggat gaactacggc agctacatgg aggagaagca catgccaccc ccaaacatga
      901 ccacgaacga gcgcagagtt atcgtgccag cagatcctac gctatggagt acagaccatg
      961 tgcggcagtg gctggagtgg gcggtgaaag aatatggcct tccagacgtc aacatcttgt
     1021 tattccagaa catcgatggg aaggaactgt gcaagatgac caaggacgac ttccagaggc
     1081 tcacccccag ctacaatgcc gacatccttc tctcacatct ccactacctc agagagactc
     1141 ctcttccaca tttgacttca gatgatgttg ataaagcctt acaaaactct ccacggttaa
     1201 tgcatgctag aaacacaggg ggtgcagctt ttattttccc aaatacttca gtatatcctg
     1261 aagctacgca aagaattaca actaggccag atttaccata tgagcccccc aggagatcag
     1321 cctggaccgg tcacggccac cccacgcccc agtcgaaagc tgctcaacca tctccttcca
     1381 cagtgcccaa aactgaagac cagcgtcctc agttagatcc ttatcagatt cttggaccaa
     1441 caagtagccg ccttgcaaat ccaggcagtg gccagatcca gctttggcag ttcctcctgg
     1501 agctcctgtc ggacagctcc aactccagct gcatcacctg ggaaggcacc aacggggagt
     1561 tcaagatgac ggatcccgac gaggtggccc ggcgctgggg agagcggaag agcaaaccca
     1621 acatgaacta cgataagctc agccgcgccc tccgttacta ctatgacaag aacatcatga
     1681 ccaaggtcca tgggaagcgc tacgcctaca agttcgactt ccacgggatc gcccaggccc
     1741 tccagcccca ccccccggag tcatctctgt acaagtaccc ctcagacctc ccgtacatgg
     1801 gctcctatca cgcccaccca cagaagatga actttgtggc gccccaccct ccagccctcc
     1861 ccgtgacatc ttccagtttt tttgctgccc caaacccata ctggaattca ccaactgggg
     1921 gtatataccc caacactagg ctccccacca gccatatgcc ttctcatctg ggcacttact
     1981 actaagaccc agctttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
     2041 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
     2101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     2701 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2761 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     2821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     3001 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3061 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3181 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3241 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3301 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3361 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3421 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3481 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3541 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4021 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4381 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4681 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4861 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5821 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5881 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5941 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6001 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6061 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6121 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6181 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6241 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6301 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6361 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6421 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     6481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6661 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6721 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6781 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6841 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6901 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6961 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7021 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7081 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7141 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7201 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7261 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7321 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7381 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7441 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7501 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7561 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7621 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7681 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7741 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7801 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7861 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7921 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt
     7981 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     8041 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     8101 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     8161 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     8221 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     8281 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     8341 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     8401 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac
     8461 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     8521 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     8581 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     8641 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8701 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8761 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8821 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga
     8881 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8941 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     9001 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     9061 agatccactt tg
//
