LOCUS       sequence-228930-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses ERG R367K (DNA binding mutant) protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Sandoval GJ, Pulice JL, Pakula H, Schenone M, Takeda DY, Pop M, 
            Boulay G, Williamson KE, McBride MJ, Pan J, St Pierre R, Hartman E, 
            Garraway LA, Carr SA, Rivera MN, Li Z, Ronco L, Hahn WC, Kadoch C
  TITLE     Binding of TMPRSS2-ERG to BAF Chromatin Remodeling Complexes 
            Mediates Prostate Oncogenesis.
  JOURNAL   Mol Cell. 2018 Aug 16;71(4):554-566.e7. doi: 
            10.1016/j.molcel.2018.06.040. Epub 2018 Aug 2.
  PUBMED    30078722
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.06.040"; journalName: "Mol Cell"; date: 
            "2018-08-16- 16"; volume: "71"; issue: "4"; pages: "554-566.e7"
COMMENT     Sequence Label: pLX304 ERG R367K
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        35..338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        339..542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     493..513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1940..1964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1966..2007
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2049..2637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2102..2122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2520..2531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2709..2942
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3020..3141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3057..3076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3111..3130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3181..3316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3336..3355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3337..3355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3365..3382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3365..3381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3374..3396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3523..3978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3610..3629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3820..3841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4004..4108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4109..4177,4178..4969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4327..4346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5882..5899
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6016..6037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6052..6057,6058..6075,6076..6082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6090..6106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6095..6117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6114..6130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6114..6130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6149..6169
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6151..6169
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6197..6423
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6424..6604
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6651..6776
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7269..7502
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7687..7731
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7906..8406
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8325..8343
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8418..8813
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8873..8990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
       61 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      541 ctctctggct aactgtcggg atcaacaagt ttgtacaaaa aagcaggctt caccatgacc
      601 gcgtcctcct ccagcgacta tggacagact tccaagatga gcccacgcgt ccctcagcag
      661 gattggctgt ctcaaccccc agccagggtc accatcaaaa tggaatgtaa ccctagccag
      721 gtgaatggct caaggaactc tcctgatgaa tgcagtgtgg ccaaaggcgg gaagatggtg
      781 ggcagcccag acaccgttgg gatgaactac ggcagctaca tggaggagaa gcacatgcca
      841 cccccaaaca tgaccacgaa cgagcgcaga gttatcgtgc cagcagatcc tacgctatgg
      901 agtacagacc atgtgcggca gtggctggag tgggcggtga aagaatatgg ccttccagac
      961 gtcaacatct tgttattcca gaacatcgat gggaaggaac tgtgcaagat gaccaaggac
     1021 gacttccaga ggctcacccc cagctacaat gccgacatcc ttctctcaca tctccactac
     1081 ctcagagaga ctcctcttcc acatttgact tcagatgatg ttgataaagc cttacaaaac
     1141 tctccacggt taatgcatgc tagaaacaca gggggtgcag cttttatttt cccaaatact
     1201 tcagtatatc ctgaagctac gcaaagaatt acaactaggc cagatttacc atatgagccc
     1261 cccaggagat cagcctggac cggtcacggc caccccacgc cccagtcgaa agctgctcaa
     1321 ccatctcctt ccacagtgcc caaaactgaa gaccagcgtc ctcagttaga tccttatcag
     1381 attcttggac caacaagtag ccgccttgca aatccaggca gtggccagat ccagctttgg
     1441 cagttcctcc tggagctcct gtcggacagc tccaactcca gctgcatcac ctgggaaggc
     1501 accaacgggg agttcaagat gacggatccc gacgaggtgg cccggcgctg gggagagcgg
     1561 aagagcaaac ccaacatgaa ctacgataag ctcagcaaag ccctccgtta ctactatgac
     1621 aagaacatca tgaccaaggt ccatgggaag cgctacgcct acaagttcga cttccacggg
     1681 atcgcccagg ccctccagcc ccaccccccg gagtcatctc tgtacaagta cccctcagac
     1741 ctcccgtaca tgggctccta tcacgcccac ccacagaaga tgaactttgt ggcgccccac
     1801 cctccagccc tccccgtgac atcttccagt ttttttgctg ccccaaaccc atactggaat
     1861 tcaccaactg ggggtatata ccccaacact aggctcccca ccagccatat gccttctcat
     1921 ctgggcactt actactaaga cccagctttc ttgtacaaag tggttggtaa gcctatccct
     1981 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
     2041 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2101 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2161 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2221 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2281 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2341 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2401 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2461 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2521 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2581 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     2641 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     2701 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     2761 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2821 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2881 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2941 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3001 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3061 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3121 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     3181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3301 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     3361 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3421 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3481 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3541 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3601 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3661 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3721 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3841 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3961 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4021 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4081 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4141 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4201 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4261 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4321 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4381 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4441 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4501 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4561 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4621 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4681 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4741 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4801 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4861 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4921 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4981 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5821 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5881 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5941 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6001 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6061 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6121 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6181 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     6241 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6301 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     6361 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     6421 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6481 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6541 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6601 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     6661 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     6721 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     6781 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     6841 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     6901 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     6961 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7021 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7081 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7141 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7201 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7261 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7321 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7381 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7441 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7501 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7561 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7621 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7681 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7741 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7801 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7861 tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa
     7921 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     7981 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     8041 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     8101 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     8161 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     8221 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     8281 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     8341 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     8401 ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     8461 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     8521 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     8581 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     8641 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     8701 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     8761 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
     8821 tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     8881 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     8941 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9001 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt tattaatagt
     9061 aatcaattac gg
//
