LOCUS       sequence-22895-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Ramadan K, Bruderer R, Spiga FM, Popp O, Baur T, Gotta M, Meyer HH
  TITLE     Cdc48/p97 promotes reformation of the nucleus by extracting the 
            kinase Aurora B from chromatin.
  JOURNAL   Nature. 2007 Dec 20. 450(7173):1258-62.
  PUBMED    18097415
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Dec 20. 450(7173):1258-62."
COMMENT     Sequence Label: pQE9-His-p97deltaD2(K251A)
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..69,70..75,76..92,93..99)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             172..189
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa
       61 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      121 caatttcaca cagaattcat taaagaggag aaattaacta tgagaggatc gcatcaccat
      181 caccatcacg gatccgcctc tggagccgat tcaaaaggtg atgatttatc aacagccatt
      241 ctcaaacaga agaaccgacc caatcggtta attgttgatg aagccatcaa tgaagataac
      301 agcgtggtgt ccttgtccca gcccaagatg gatgaactgc agttgttccg aggtgacacg
      361 gtgttgctaa aaggaaagaa aagacgggaa gctgtatgca ttgttctttc tgatgacacg
      421 tgttctgatg agaagattcg aatgaataga gttgttcgga ataacctccg agttcgccta
      481 ggagatgtca tcagcatcca gccatgccct gatgtaaagt atggcaaacg tatccacgtt
      541 ctacccatcg atgacacagt ggaaggcatc actggcaatc tctttgaggt ataccttaag
      601 ccgtacttcc tggaagctta tcggcccatc cgtaaaggag atatttttct tgtccggggt
      661 gggatgcgtg ctgtggagtt caaagttgta gagacagatc ccagccctta ctgtattgtt
      721 gctccagaca cagtgatcca ctgtgagggg gagccaatca aacgag
//
