LOCUS       sequence-228951-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses control HA-birA* in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-STOP-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             157..183
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcta ccatggcata acgactaata tttatgtacc catacgatgt gcctgactat
      181 gctaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
      241 tctggcgagc agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc
      301 cagacactga gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg
      361 cctgagccta tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc
      421 gtggccgtgc tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag
      481 ctgaagtccg gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga
      541 cggaagtggt tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag
      601 cagggacctg ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg
      661 ctgagaaagc tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag
      721 gaccgcaagc tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag
      781 atcgtgatcg gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac
      841 cagggctgga tcaccctgca gga
//
