LOCUS       sequence-228956-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..637
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(844..861)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gggcggagaa atctccctga gaagcgcaga gaagggatcc agcggcggcg gcagcggcgg
       61 cggcggcagc acggaatata agctggtggt ggtgggcgcc ggcggtgtgg gcaagagtgc
      121 gctgaccatc cagctgatcc agaaccattt tgtggacgaa tacgacccca ctatagagga
      181 ttcctaccgg aagcaggtgg tcattgatgg ggagacgtgc ctgttggaca tcctggatac
      241 cgccggccag gaggagtaca gcgccatgcg ggaccagtac atgcgcaccg gggagggctt
      301 cctgtgtgtg tttgccatca acaacaccaa gtcttttgag gacatccacc agtacaggga
      361 gcagatcaaa cgggtgaagg actcggatga cgtgcccatg gtgctggtgg ggaacaagtg
      421 tgacctggct gcacgcactg tggaatctcg gcaggctcag gacctcgccc gaagctacgg
      481 catcccctac atcgagacct cggccaagac ccggcaggga gtggaggatg ccttctacac
      541 gttggtgcgt gagatccggc agcacaagct gcggaagctg aaccctcctg atgagagtgg
      601 ccccggctgc atgagctgca agtgtgtgct ctcctgactc gagtctagag ggcccgacgc
      661 gtctggccac cggtcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
      721 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
      781 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
      841 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaa
//
