LOCUS       sequence-228962-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(60..89,90..92,93..626)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(833..850)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tctccctgag aagcgcagag aagggatcca gcggcggcgg cagcggcggc ggcggcagca
       61 cggaatataa gctggtggtg gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc
      121 agctgatcca gaaccatttt gtggacgaat acgaccccac tatagaggat tcctaccgga
      181 agcaggtggt cattgatggg gagacgtgcc tgttggacat cctggatacc gccggccagg
      241 aggagtacag cgccatgcgg gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt
      301 ttgccatcaa caacaccaag tcttttgagg acatccacca gtacagggag cagatcaaac
      361 gggtgaagga ctcggatgac gtgcccatgg tgctggtggg gaacaagtgt gacctggctg
      421 cacgcactgt ggaatctcgg caggctcagg acctcgcccg aagctacggc atcccctaca
      481 tcgagacctc ggccaagacc cggcagggag tggaggatgc cttctacacg ttggtgcgtg
      541 agatccggca gcacaagctg cggaagctga accctcctga tgagagtggc cccggctgca
      601 tgagctgcaa gtgtgtgctc tcctgactcg agtctagagg gcccgacgcg tctggccacc
      661 ggtcgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      721 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      781 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      841 aaaggaatgc a
//
