LOCUS       sequence-228965-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             137..163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctac
      121 tagaggatct accatgtacc catacgatgt gcctgactat gctaaggaca acaccgtgcc
      181 cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc agctgggaga
      241 gaccctggga atgagcagag ccgccatcaa caagcacatc cagacactga gagactgggg
      301 agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta tccagctgct
      361 gaacgccaag cagatcctgg gacagctgga tggcggaagc gtggccgtgc tgcctgtgat
      421 cgactccacc aatcagtacc tgctggacag aatcggagag ctgaagtccg gcgacgcctg
      481 catcgccgag taccagcagg ctggcagagg aggcagagga cggaagtggt tcagcccatt
      541 cggagccaac ctgtacctgt ccatgttctg gagactggag cagggacctg ctgctgccat
      601 cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc tgggagccga
      661 caaggtgaga gtgaagtggc ctaatgacct gtacctccag gaccgcaagc tggctggcat
      721 cctggtggag ctgacaggca agacaggcga tgccgctcag atcgtgatcg gagccggaat
      781 caacatggcc atgagaagag tggaggagag cgtggtgaac cagggctgga tcaccctgca
      841 ggaggctggc atcaacctgg accggaacac cctggccgcc atgctgatca
//
