LOCUS       sequence-228967-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..671
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 tgctgctgga gcaggacgga atcatcaagc cctggatggg cggagaaatc tccctgagaa
       61 gcgcagagaa gggatccagc ggcggcggca gcggcggcgg cggcagcact gaatataaac
      121 ttgtggtagt tggagctggt ggcgtaggca agagtgcctt gacgatacag ctaattcaga
      181 atcattttgt ggacgaatat gatccaacaa tagaggattc ctacaggaag caagtagtaa
      241 ttgatggaga aacctgtctc ttggatattc tcgacacagc aggtcaagag gagtacagtg
      301 caatgaggga ccagtacatg aggactgggg agggctttct ttgtgtattt gccataaata
      361 atactaaatc atttgaagat attcaccatt atagagaaca aattaaaaga gttaaggact
      421 ctgaagatgt acctatggtc ctagtaggaa ataaatgtga tttgccttcc agaacagtag
      481 acacaaaaca ggctcaggac ttagcaagaa gttatggaat tccttttatt gaaacatcag
      541 caaagacaag acagggtgtt gatgatgcct tctatacatt agttcgagaa attcgaaaac
      601 ataaagaaaa gatgagcaaa gatggtaaaa agaagaaaaa gaagtcaaag acaaagtgtg
      661 taattatgta actcgagtct agagggcccg acgcgtctgg ccaccggtcg cc
//
