LOCUS       sequence-228969-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             136..162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcta ccatgtaccc atacgatgtg cctgactatg ctaaggacaa caccgtgccc
      181 ctgaagctga tcgccctgct ggccaacggc gagttccact ctggcgagca gctgggagag
      241 accctgggaa tgagcagagc cgccatcaac aagcacatcc agacactgag agactgggga
      301 gtggacgtgt tcaccgtgcc tggcaagggc tacagcctgc ctgagcctat ccagctgctg
      361 aacgccaagc agatcctggg acagctggat ggcggaagcg tggccgtgct gcctgtgatc
      421 gactccacca atcagtacct gctggacaga atcggagagc tgaagtccgg cgacgcctgc
      481 atcgccgagt accagcaggc tggcagagga ggcagaggac ggaagtggtt cagcccattc
      541 ggagccaacc tgtacctgtc catgttctgg agactggagc agggacctgc tgctgccatc
      601 ggactgagtc tggtgatcgg aatcgtgatg gccgaggtgc tgagaaagct gggagccgac
      661 aaggtgagag tgaagtggcc taatgacctg tacctccagg accgcaagct ggctggcatc
      721 ctggtggagc tgacaggcaa gacaggcgat gccgctcaga tcgtgatcgg agccggaatc
      781 aacatggcc
//
