LOCUS       sequence-228970-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(71..100,101..103,104..634)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             71..634
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(841..858)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gggcggagaa atctccctga gaagcgcaga gaagggatcc agcggcggcg gcagcggcgg
       61 cggcggcagc actgaatata aacttgtggt agttggagct gatggcgtag gcaagagtgc
      121 cttgacgata cagctaattc agaatcattt tgtggacgaa tatgatccaa caatagagga
      181 ttcctacagg aagcaagtag taattgatgg agaaacctgt ctcttggata ttctcgacac
      241 agcaggtcaa gaggagtaca gtgcaatgag ggaccagtac atgaggactg gggagggctt
      301 tctttgtgta tttgccataa ataatactaa atcatttgaa gatattcacc attatagaga
      361 acaaattaaa agagttaagg actctgaaga tgtacctatg gtcctagtag gaaataaatg
      421 tgatttgcct tccagaacag tagacacaaa acaggctcag gacttagcaa gaagttatgg
      481 aattcctttt attgaaacat cagcaaagac aagacagggt gttgatgatg ccttctatac
      541 attagttcga gaaattcgaa aacataaaga aaagatgagc aaagatggta aaaagaagaa
      601 aaagaagtca aagacaaagt gtgtaattat gtaactcgag tctagagggc ccgacgcgtc
      661 tggccaccgg tcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
      721 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
      781 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
      841 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
      901 cttcttgaag acaaacaacg tctgtagcga ccctttgc
//
