LOCUS       sequence-228971-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(221..250,251..253,254..784)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             221..784
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 ttacctgagc agatgggaga agctggacaa cttcatcaac agacctgtga agctgatcat
       61 cggcgacaag gaaatcttcg gcatctccag aggaatcgac aagcagggag ctctgctgct
      121 ggagcaggac ggaatcatca agccctggat gggcggagaa atctccctga gaagcgcaga
      181 gaagggatcc agcggcggcg gcagcggcgg cggcggcagc actgaatata aacttgtggt
      241 agttggagct gatggcgtag gcaagagtgc cttgacgata cagctaattc agaatcattt
      301 tgtggacgaa tatgatccaa caatagagga ttcctacagg aagcaagtag taattgatgg
      361 agaaacctgt ctcttggata ttctcgacac agcaggtcaa gaggagtaca gtgcaatgag
      421 ggaccagtac atgaggactg gggagggctt tctttgtgta tttgccataa ataatactaa
      481 atcatttgaa gatattcacc attatagaga acaaattaaa agagttaagg actctgaaga
      541 tgtacctatg gtcctagtag gaaataaatg tgatttgcct tccagaacag tagacacaaa
      601 acaggctcag gacttagcaa gaagttatgg aattcctttt attgaaacat cagcaaagac
      661 aagacagggt gttgatgatg ccttctatac attagttcga gaaattcgaa aacataaaga
      721 aaagatgagc aaagatggta aaaagaagaa aaagaagtca aagacaaagt gtgtaattat
      781 gtaactcgag tctagagggc ccgacgcgtc tggccaccgg tcgcccctct ccctcccccc
      841 cccc
//
