LOCUS       sequence-228974-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(840..857)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggagaaatct ccctgagaag cgcagagaag ggatccagcg gcggcggcag cggcggcggc
       61 ggcagcactg agtacaaact ggtggtggtt ggagcaggtg gtgttgggaa aagcgcactg
      121 acaatccagc taatccagaa ccactttgta gatgaatatg atcccaccat agaggattct
      181 tacagaaaac aagtggttat agatggtgaa acctgtttgt tggacatact ggatacagct
      241 ggacaagaag agtacagtgc catgagagac caatacatga ggacaggcga aggcttcctc
      301 tgtgtatttg ccatcaataa tagcaagtca tttgcggata ttaacctcta cagggagcag
      361 attaagcgag taaaagactc ggatgatgta cctatggtgc tagtgggaaa caagtgtgat
      421 ttgccaacaa ggacagttga tacaaaacaa gcccacgaac tggccaagag ttacgggatt
      481 ccattcattg aaacctcagc caagaccaga cagggtgttg aagatgcttt ttacacactg
      541 gtaagagaaa tacgccagta ccgaatgaaa aaactcaaca gcagtgatga tgggactcag
      601 ggttgtatgg gattgccatg tgtggtgatg taactcgagt ctagagggcc cgacgcgtct
      661 ggccaccggt cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
      721 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
      781 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
      841 tctcgccaaa ggaatgcaag gtctgttgaa tg
//
