LOCUS       sequence-228977-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..44
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             136..162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
       61 cacgctgttt tgacctccat agaagacacc gactctacta gaggatctac catggcataa
      121 cgactaatat ttatgtaccc atacgatgtg cctgactatg ctaaggacaa caccgtgccc
      181 ctgaagctga tcgccctgct ggccaacggc gagttccact ctggcgagca gctgggagag
      241 accctgggaa tgagcagagc cgccatcaac aagcacatcc agacactgag agactgggga
      301 gtggacgtgt tcaccgtgcc tggcaagggc tacagcctgc ctgagcctat ccagctgctg
      361 aacgccaagc agatcctggg acagctggat ggcggaagcg tggccgtgct gcctgtgatc
      421 gactccacca atcagtacct gctggacaga atcggagagc tgaagtccgg cgacgcctgc
      481 atcgccgagt accagcaggc tggcagagga ggcagaggac ggaagtggtt cagcccattc
      541 ggagccaacc tgtacctgtc catgttctgg agactggagc agggacctgc tgctgccatc
      601 ggactgagtc tggtgatcgg aatcgtgatg gccgaggtgc tgagaaagct gggagccgac
      661 aaggtgagag tgaagtggcc taatgacctg tacctccagg accgcaagct ggctggcatc
      721 ctggtggagc tgacaggcaa gacaggcgat gccgctcaga tcgtgatcgg agccggaatc
      781 aacatggcca tgagaagagt ggaggagagc gtggtgaacc agggctggat caccctgcag
//
