LOCUS       sequence-22898-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for N-terminal 3x HA tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE2n
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             140..166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             140..166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             167..193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             167..193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             209..235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             209..235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(306..405)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(517..536)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(595..614)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctactgggcc aatatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa
      121 ttcagtcgac atgtcgcgat acccctacga cgtgcccgac tacgcctacc cctacgacgt
      181 gcccgactac gccgatcgat ccggaccgta cccctacgac gtgcccgact acgccgctag
      241 cagtggagga tccggtacag atttcggaca tgcggccgca taagtagctg actcgagata
      301 tctagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      361 ttgcaacgaa caggtcacta tcngtcaaaa taaaatcatt atttgncntc cngntgcagn
      421 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      481 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
      541 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
      601 aatgggctcg cgataatgtc gggctntcag gtgcgacaat ctatcncttt gatgggaagc
      661 ccgatgcccc anagttgttt nggaaacatt ta
//
