LOCUS       sequence-228981-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             158..184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctac
      121 tagaggatct accatggcat aacgactaat atttatgtac ccatacgatg tgcctgacta
      181 tgctaaggac aacaccgtgc ccctgaagct gatcgccctg ctggccaacg gcgagttcca
      241 ctctggcgag cagctgggag agaccctggg aatgagcaga gccgccatca acaagcacat
      301 ccagacactg agagactggg gagtggacgt gttcaccgtg cctggcaagg gctacagcct
      361 gcctgagcct atccagctgc tgaacgccaa gcagatcctg ggacagctgg atggcggaag
      421 cgtggccgtg ctgcctgtga tcgactccac caatcagtac ctgctggaca gaatcggaga
      481 gctgaagtcc ggcgacgcct gcatcgccga gtaccagcag gctggcagag gaggcagagg
      541 acggaagtgg ttcagcccat tcggagccaa cctgtacctg tccatgttct ggagactgga
      601 gcagggacct gctgctgcca tcggactgag tctggtgatc ggaatcgtga tggccgaggt
      661 gctgagaaag ctgggagccg acaaggtgag agtgaagtgg cctaatgacc tgtacctcca
      721 ggaccgcaag ctggctggca tcctggtgga gctgacaggc aagacaggcg atgccgctca
      781 gatcgtgatc ggagccggaa tcaacatggc c
//
