LOCUS       sequence-228982-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(832..849)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctccctgaga agcgcagaga agggatccag cggcggcggc agcggcggcg gcggcagcac
       61 tgagtacaaa ctggtggtgg ttggagcagg tggtgttggg aaaagcgcac tgacaatcca
      121 gctaatccag aaccactttg tagatgaata tgatcccgcc atagaggatt cttacagaaa
      181 acaagtggtt atagatggtg aaacctgttt gttggacata ctggatacag ctggaaaaga
      241 agagtacagt gccatgagag accaatacat gaggacaggc gaaggcttcc tctgtgtatt
      301 tgccatcaat aatagcaagt catttgcgga tattaacctc tacagggagc agattaagcg
      361 agtaaaagac tcggatgatg tacctatggt gctagtggga aacaagtgtg atttgccaac
      421 aaggacagtt gatacaaaac aagcccacga actggccaag agttacggga ttccattcat
      481 tgaaacctca gccaagacca gacagggtgt tgaagatgct ttttacacac tggtaagaga
      541 aatacgccag taccgaatga aaaaactcaa cagcagtgat gatgggactc agggttgtat
      601 gggattgcca tgtgtggtga tgtaactcga gtctagaggg cccgacgcgt ctggccaccg
      661 gtcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
      721 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
      781 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
      841 aaggaatgca agg
//
