LOCUS       sequence-228983-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctgctgctg gagcaggacg gaatcatcaa gccctggatg ggcggagaaa tctccctgag
       61 aagcgcagag aagggatcca gcggcggcgg cagcggcggc ggcggcagca ctgagtacaa
      121 actggtggtg gttggagcag gtggtgttgg gaaaagcgca ctgacaatcc agctaatcca
      181 gaaccacttt gtagatgaat atgatcccgc catagaggat tcttacagaa aacaagtggt
      241 tatagatggt gaaacctgtt tgttggacat actggataca gctggaaaag aagagtacag
      301 tgccatgaga gaccaataca tgaggacagg cgaaggcttc ctctgtgtat ttgccatcaa
      361 taatagcaag tcatttgcgg atattaacct ctacagggag cagattaagc gagtaaaaga
      421 ctcggatgat gtacctatgg tgctagtggg aaacaagtgt gatttgccaa caaggacagt
      481 tgatacaaaa caagcccacg aactggccaa gagttacggg attccattca ttgaaacctc
      541 agccaagacc agacagggtg ttgaagatgc tttttacaca ctggtaagag aaatacgcca
      601 gtaccgaatg aaaaaactca acagcagtga tgatgggact cagggttgta tgggattgcc
      661 atgtgtggtg atgtaactcg agtctagagg gcccgacgcg tctggccacc ggtcgcccct
      721 ctccctcccc ccccc
//
